Research in the Sheffield lab
Nathan Sheffield, PhD
We develop and apply computational methods
to organize, analyze, and understand large epigenomic data.
Every cell in your body has the same genome*...
Yet your cells are diverse
How can a single set of instructions
give rise to such a complex multicellular being?
Through the process of differentiation,
cells assume individual functions and roles
But when differentiation goes awry, disease may result
Each cell-type folds DNA differently,
thereby using a different subset and
providing a molecular signature of its behavior
DNA folding can be measured by sequencing DNA
But huge, diverse datasets lead to computational challenges.
Defining cell type from chromatin accessibility
Sheffield et al. (2013).
Epigenetic heterogeneity in pediatric cancer
Sheffield et al. (2017).
Inferring regulatory activity from DNA methylation
Lawson et al. (2018).
Connecting the genome to the epigenome
Acute Myeloid Leukemia epigenomics
Standardizing metadata for big biomedical data analysis
Thanks for listening!